癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DEPTOR Antibody
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Application 
| WB, E, IHC-P |
|---|---|
| Primary Accession | Q8TB45 |
| Other Accession | NP_073620, 189571664 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 46294 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. |
| Gene ID | 64798 |
|---|---|
| Other Names | DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6, DEPTOR, DEPDC6 |
| Target/Specificity | DEPTOR; DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms. |
| Reconstitution & Storage | DEPTOR antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | DEPTOR Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | DEPTOR {ECO:0000303|PubMed:19446321, ECO:0000312|HGNC:HGNC:22953} |
|---|---|
| Function | Negative regulator of the mTORC1 and mTORC2 complexes: inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed:19446321, PubMed:22017875, PubMed:22017876, PubMed:22017877, PubMed:25936805, PubMed:29382726, PubMed:34519268, PubMed:34519269). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed:22017875, PubMed:22017876, PubMed:22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed:34519268, PubMed:34519269). |
| Cellular Location | Lysosome membrane. Note=Localizes to the lysosomal membrane when associated with the mTORC1 and mTORC2 complexes. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
REFERENCES
Peterson TR, Laplante M, Thoreen CC, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for survival. Cell 2009; 137:873-6.
Duan S, Skaar JR, Kuchay S, et al. mTOR generates an auto-amplification loop by triggering the bTrCP- and CK1a-dependent degradation of DEPTOR. Mol. Cell 2011; 44:317-24.
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