癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
WAPL Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q7Z5K2 |
| Other Accession | NP_055860, 42734325 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 132946 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL. |
| Gene ID | 23063 |
|---|---|
| Other Names | Wings apart-like protein homolog, Friend of EBNA2 protein, WAPAL, FOE, KIAA0261, WAPL |
| Target/Specificity | WAPAL; WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist. |
| Reconstitution & Storage | WAPL antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | WAPL Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | WAPL (HGNC:23293) |
|---|---|
| Synonyms | FOE, KIAA0261, WAPAL |
| Function | Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin (PubMed:26299517). Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. |
| Cellular Location | [Isoform 2]: Nucleus. |
| Tissue Location | Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1). It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1,2,4). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3,4). WAPL is a new regulator of the development and metastasis of cancerous tissue (4,5).
REFERENCES
Kwiatkowski BA, Ragoczy T, Ehly J, et al. Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Exp. Cell Res. 2004; 300:223-33.
Kueng S, Hegemann B, Peters BH, et al. Wapl controls the dynamic association of cohesion with chromatin. Cell 2006; 127:955-67.
Gandhi R, Gillespie PJ, and Hirano T. Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Curr. Biol. 2006; 16:2406-17.
Oikawa K, Ohbayashi T, Kiyono T, et al. Expression of a novel human gene, human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression. Cancer Res. 2004; 64:3545-9.
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