癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SREBF1 Antibody
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- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | P36956 |
| Other Accession | NP_001005291, 52630419 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 121675 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SREBF1 antibody can be used for detection of SREBF1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL. |
| Gene ID | 6720 |
|---|---|
| Other Names | Sterol regulatory element-binding protein 1, SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1, Processed sterol regulatory element-binding protein 1, SREBF1, BHLHD1, SREBP1 |
| Target/Specificity | SREBF1; SREBF1 antibody is human and mouse reactive. At least three isoforms of SREBF1 are known to exist. SREBF1 antibody is predicted not to cross-react with SREBF2. |
| Reconstitution & Storage | SREBF1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | SREBF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SREBF1 {ECO:0000303|PubMed:7759101, ECO:0000312|HGNC:HGNC:11289} |
|---|---|
| Function | [Sterol regulatory element-binding protein 1]: Precursor of the transcription factor form (Processed sterol regulatory element- binding protein 1), which is embedded in the endoplasmic reticulum membrane (PubMed:32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis (By similarity). |
| Cellular Location | [Sterol regulatory element-binding protein 1]: Endoplasmic reticulum membrane; Multi- pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane {ECO:0000250|UniProtKB:Q9WTN3}; Multi-pass membrane protein. Note=At high sterol concentrations, the SCAP-SREBP is retained in the endoplasmic reticulum. Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed {ECO:0000250|UniProtKB:Q9WTN3} [Isoform SREBP-1aDelta]: Nucleus |
| Tissue Location | Expressed in a wide variety of tissues, most abundant in liver and adrenal gland (PubMed:8402897). In fetal tissues lung and liver shows highest expression (PubMed:8402897) [Isoform SREBP-1C]: Predominantly expressed in liver and adipose tissues (PubMed:8402897). Also expressed in kidney, brain, white fat, and muscle (PubMed:8402897) |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The sterol regulatory element binding transcription factor 1 (SREBF1) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1). The related protein SREBF2 also binds SRE1 and activates transcription in an additive fashion to SREBF1 (2). SREBF1 is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1 (3). The SREBF1 proteins are important in the regulation of genes involved in lipid metabolism, while SREBF2 has been more closely associated with cholesterol synthesis and accumulation (4).
REFERENCES
Wang X, Briggs MR, Hua X, et al. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. II. Purification and characterization. J. Biol. Chem. 1993; 268:14497-504.
Hua X, Yokoyama C, Wu J, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA 1993; 90:11603-7.
Wang X, Sato R, Brown MS, et al. SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis. Cell 1994; 77:53-62.
Raghow R, Yellaturu C, Deng X, et al. SREBPs: the crossroads of physiological and pathological homeostasis. Endocrinol. Metab. 2008; 19:65-73.
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