癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
AKAP4 Antibody
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- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | Q5JQC9 |
| Other Accession | NP_003877, 21493037 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 94477 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 8852 |
|---|---|
| Other Names | A-kinase anchor protein 4, AKAP-4, A-kinase anchor protein 82 kDa, AKAP 82, hAKAP82, Major sperm fibrous sheath protein, HI, Protein kinase A-anchoring protein 4, PRKA4, AKAP4 (HGNC:374), AKAP82 |
| Target/Specificity | AKAP4; AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms. |
| Reconstitution & Storage | AKAP4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | AKAP4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AKAP4 (HGNC:374) |
|---|---|
| Synonyms | AKAP82 |
| Function | Major structural component of sperm fibrous sheath (PubMed:9822690). Plays a role in sperm motility (PubMed:34415320, PubMed:9822690). |
| Cellular Location | Cell projection, cilium, flagellum. Note=Localizes to the principle piece of the sperm flagellum. |
| Tissue Location | Testis specific; only expressed in round spermatids. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1). AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm (2). It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways (2). AKAP4 has also been postulated to be a potential biomarker for breast cancer (3).
REFERENCES
Carnegie GK, Means CK, and Scott JD. A-kinase anchoring proteins: from protein complexes to physiology and disease. IUBMB 2009; 61:394-406.
Turner RM, Johnson LR, Haig-Ladewig L, et al. An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail. J. Biol. Chem. 1998; 273:32135-41.
Saini S, Jagadish N, Gupta A, et al. A novel cancer testis antigen, A-kinase anchor protein 4 (AKAP4) is a potential biomarker for breast cancer. PLoS One 2013; 8:e57095.
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