癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Beclin 2 Antibody
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- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | A8MW95 |
| Other Accession | NP_001277622, 595582373 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 48153 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | Beclin 2 antibody can be used for detection of Beclin 2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 441925 |
|---|---|
| Other Names | Beclin-2, Beclin-1 autophagy-related pseudogene 1 {ECO:0000312|HGNC:HGNC:38606}, Beclin-1-like protein 1 {ECO:0000312|MIM:615687}, BECN1P1 (HGNC:38606) |
| Target/Specificity | BECN1P1; Beclin 2 antibody is human specific. At least two isoforms of Beclin 2 are known to exist; this antibody will only detect the longer isoform. Beclin 2 antibody is predicted to not cross-react with Beclin 1. |
| Reconstitution & Storage | Beclin 2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | Beclin 2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BECN2 {ECO:0000303|PubMed:23954414, ECO:0000312|HGNC:HGNC:38606} |
|---|---|
| Function | Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1. |
| Cellular Location | Cytoplasm. |
| Tissue Location | Present in fetal and adult brain (at protein level). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin 2 is a mammalian specific homolog of the autophagy protein Beclin 1 (3). Like Beclin 1, Beclin 2 interacts with Bcl-2 and class III PI3K complex components. However, Beclin 2 functions in an additional lysosomal degradation pathway and is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (3). Beclin 2 is also required for agonist-induced lysosome-mediated degradation of EGFR in lung cancer cells, suggesting that it may also play a role in regulating other intracellular signaling pathways (4).
REFERENCES
Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
He C, Wei Y, Sun K, et al. Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism. Cell 2013; 154:1085-99.
Zhang W and He C. Regulation of plasma membrane receptors by a new autophagy-related BECN/Beclin family member. Autophagy 2014; 10: epub.
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