癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SLC1A7 Antibody
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- 实验流程
- 背景知识
Application 
| WB, IF, E, IHC-P |
|---|---|
| Primary Accession | O00341 |
| Other Accession | NP_001274524, 567316146 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 60658 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 6512 |
|---|---|
| Other Names | Excitatory amino acid transporter 5, Retinal glutamate transporter, Solute carrier family 1 member 7, SLC1A7, EAAT5 |
| Target/Specificity | SLC1A7; SLC1A7 antibody is human and mouse reactive. Multiple isoforms of SLC1A7 are known to exist. |
| Reconstitution & Storage | SLC1A7 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | SLC1A7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | SLC1A7 (HGNC:10945) |
|---|---|
| Synonyms | EAAT5 |
| Function | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D- aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:9108121). Acts primarily as an inhibitory glutamate-gated chloride channel being a major inhibitory presynaptic receptor at mammalian rod bipolar cell axon terminals. Glutamate binding gates a large Cl(-) conductance that mediates inhibition, affecting visual processing in the retina (By similarity). |
| Cellular Location | Photoreceptor inner segment membrane {ECO:0000250|UniProtKB:Q8JZR4}; Multi-pass membrane protein. Synaptic cell membrane {ECO:0000250|UniProtKB:Q8JZR4}; Multi-pass membrane protein. Note=Located in both cone and rod photoreceptor terminals and in axon terminals of rod bipolar cells. {ECO:0000250|UniProtKB:Q8JZR4} |
| Tissue Location | Expressed primarily in retina. Detectable in liver, heart, muscle and brain. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The solute carrier family 1 member 7 (SLC1A7) protein was initially identified as a glutamate transporter coupled to a chloride conductance that is expressed primarily in the retina (1). More recent evidence indicates that is it widely expressed in peripheral tissues, also (2). Experiments have shown that SLC1A7 functions as a low-affinity/low-capacity glutamate transport, with an anion channel optimized for anion conductance in the negative voltage range (3). In the retina, SLC1A7 co-localizes with the serum and glucocorticoid dependent kinase SGK1, and its cell surface expression and activity is regulated SGK1 and its isoform SGK3 (4).
REFERENCES
Arriza JL, Eliasof S, Kavanaugh MP, et al. Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc. Natl. Acad. Sci. USA 1997; 94:4155-60.
Lee A, Anderson AR, Stevens M, et al. Excitatory amino acid transporter 5 is widely expressed in peripheral tissues. Eur. J. Histochem. 2013; 57:e11.
Schneider N, Cordeiro S, Machtens JP, et al. Functional properties of the retinal glutamate transporters GLT-1c and EAAT5. J. Biol. Chem. 2014; 289:1815-24.
Boehmer C, Rajamanickam J, Schniepp R, et al. Regulation of the excitatory amino acid transporter EAAT5 by the serum and glucocorticoid dependent kinases SGK1 and SGK3. Biochem. Biophys. Res. Commun. 2005; 329:738-42.
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