癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GLIS2 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E, IHC-P |
|---|---|
| Primary Accession | Q9BZE0 |
| Other Accession | NP_115964, 110431364 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 55689 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | GLIS2 antibody can be used for detection of GLIS2 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. |
| Gene ID | 84662 |
|---|---|
| Other Names | Zinc finger protein GLIS2, GLI-similar 2, Neuronal Krueppel-like protein, GLIS2, NKL |
| Target/Specificity | GLIS2; GLIS2 antibody is human, mouse and rat reactive. |
| Reconstitution & Storage | GLIS2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | GLIS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | GLIS2 |
|---|---|
| Synonyms | NKL |
| Function | Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway (By similarity). Represses the Hedgehog-dependent expression of Wnt4 (By similarity). Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to- mesenchymal transition (By similarity). Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation (By similarity). |
| Cellular Location | Nucleus speckle. Cytoplasm |
| Tissue Location | Expressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
GLIS2, also known as neuronal Krueppel-like, is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family (1). GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation (1,2). GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta. Mutations of GLIS2 have been suggested to be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis (3,4).
REFERENCES
Vasanth S, ZeRuth G, Kang HS, et al. Identification of nuclear localization, DNA binding, and transactivating mechanisms of Kruppel-like zinc finger protein Gli-similar 2 (Glis2). J. Biol. Chem. 2011; 286:4749-59.
Hosking CR, Ulloa F, Hogan C, et al. The transcriptional repressor Glis2 is a novel binding partner for p120 catenin. Mol. Biol. Cell 2007; 18:1918-27.
Kim YS, Kang HS, Herbert R, et al. Kruppel-like zinc finger protein Glis2 is essential for the maintenance of normal renal functions. Mol. Cell Biol. 2008; 28:2358-67.
Attanasio M, Uhlenhaut NH, Sousa VH, et al. Loss of GLIS2 causes nephronophthisis in humans and mice by increased apoptosis and fibrosis. Nat. Genet. 2007; 39:1018-24.
终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问，联系：0512-88856768 tech-china@abcepta.com.
