癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DNAJC13 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E, IHC-P |
|---|---|
| Primary Accession | O75165 |
| Other Accession | NP_056083, 112421122 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 254415 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. |
| Gene ID | 23317 |
|---|---|
| Other Names | DnaJ homolog subfamily C member 13, Required for receptor-mediated endocytosis 8, RME-8, DNAJC13, KIAA0678, RME8 |
| Target/Specificity | DNAJC13; DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members. |
| Reconstitution & Storage | DNAJC13 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | DNAJC13 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | DNAJC13 |
|---|---|
| Synonyms | KIAA0678, RME8 |
| Function | Involved in membrane trafficking through early endosomes, such as the early endosome to recycling endosome transport implicated in the recycling of transferrin and the early endosome to late endosome transport implicated in degradation of EGF and EGFR (PubMed:18256511, PubMed:18307993). Involved in the regulation of endosomal membrane tubulation and regulates the dynamics of SNX1 on the endosomal membrane; via association with WASHC2 may link the WASH complex to the retromer SNX-BAR subcomplex (PubMed:24643499). |
| Cellular Location | Early endosome. Early endosome membrane; Peripheral membrane protein. Endosome membrane |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).
REFERENCES
Girard M, Poupon V, Blondeau F, et al. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J. Biol. Chem. 2005; 280:40135-43.
Fujibayashi A, Taguchi T, Misaki R, et al. Human RME-8 is involved in membrane trafficking through early endosomes. Cell Struct. Funct. 2008; 33:35-50.
Girard M and McPherson PS. RME-8 regulates trafficking of the epidermal growth factor receptor. FEBS Lett. 2008; 582:961-6.
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