癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PACS1 Antibody
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q6VY07 |
| Other Accession | NP_060496, 30089916 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 104898 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | PACS1 antibody can be used for detection of PACS1 by Western blot at 1 - 2 µg/ml. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 55690 |
|---|---|
| Other Names | Phosphofurin acidic cluster sorting protein 1, PACS-1, PACS1, KIAA1175 |
| Target/Specificity | PACS1; PACS1 antibody is human, mouse and rat reactive. Multiple isoforms of PACS1 are known to exist. PACS1 antibody is predicted to not cross-react with PACS2. |
| Reconstitution & Storage | PACS1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | PACS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PACS1 |
|---|---|
| Synonyms | KIAA1175 |
| Function | Coat protein that is involved in the localization of trans- Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster- containing cytoplasmic domain of these molecules with the adapter- protein complex-1 (AP-1) of endosomal clathrin-coated membrane pits. Involved in HIV-1 nef-mediated removal of MHC-I from the cell surface to the TGN. Required for normal ER Ca2+ handling in lymphocytes. Together with WDR37, it plays an essential role in lymphocyte development, quiescence and survival. Required for stabilizing peripheral lymphocyte populations (By similarity). |
| Cellular Location | Golgi apparatus, trans-Golgi network {ECO:0000250|UniProtKB:O88588}. Note=Localizes in the perinuclear region, probably the TGN. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The phosphofurin acidic cluster sorting protein 1 (PACS1), belongs to the PACS family of cytosolic connector proteins that localize different membrane proteins to the trans-Golgi network (TGN) (1,2). Mutations in PACS1 cause defects in cranial-neural crest migration during early development, leading to a recognizable intellectual disability syndrome (3). PACS1, along with the related protein PACS2, also plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance (4).
REFERENCES
Wan L, Molloy SS, Thomas L, et al. PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization. Cell 1998; 94:205-16.
Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16.
Schuurs-Hoeijmakers JH, Oh EC, Vissers LE, et al. Recurrent de novo mutations in PACS1 cause defective cranial-neural-crest migration and define a recognizable intellectual-disability syndrome. Am. J. Hum. Genet. 2012; 91:1122-7.
Dikeakos JD, Thomas L, Kwon G, et al. An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS2 on endosomes to down-regulate MHC-I. Mol. Biol. Cell 2012; 23:2184-97.
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