癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
IZUMO1 Antibody
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- 背景知识
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | Q8IYV9 |
| Other Accession | NP_872381, 194097475 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 38930 Da |
| Concentration (mg/ml) | 1 mg/mL |
| Conjugate | Unconjugated |
| Application Notes | IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. |
| Gene ID | 284359 |
|---|---|
| Other Names | Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo, IZUMO1 |
| Target/Specificity | IZUMO1; IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms. |
| Reconstitution & Storage | IZUMO1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | IZUMO1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | IZUMO1 {ECO:0000303|PubMed:15759005, ECO:0000312|HGNC:HGNC:28539} |
|---|---|
| Function | Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human- specific oolemma epitope FCRL3/MAIA during fertilization (PubMed:36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed:36070373). Plays a critical role in sperm-oolemma binding prior to plasma membrane fusion. Can mediate cell-cell fusion in cultured mammalian cells independently of its binding to IZUMO1R/JUNO (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type I membrane protein. Note=Localizes initially to the acrosome membrane of the sperm head (both outer and inner acrosomal membranes) (PubMed:36070373). During the acrosome reaction, translocates to the plasma membrane (PubMed:15759005, PubMed:36070373). |
| Tissue Location | Sperm-specific (at protein level) (PubMed:15759005, PubMed:36070373). Detectable on sperm surface only after the acrosome reaction (PubMed:15759005, PubMed:36070373) |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4).
REFERENCES
Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140:3221-9.
Inoue N, Ikawa M, and Okabe M. The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion. Asian J. Androl. 2011; 13:81-7.
Xing WJ, Han BD, Wu Q, et al. Molecular cloning and characterization of Izumo1 gene from sheep and cashmere goat reveal alternative splicing. Mol. Biol. Rep. 2011; 38:1995-2006.
Inoue N, Ikawa M, Isotani A, et al. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 2005; 434:234-8.
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