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SARS-CoV-2 (COVID-19) Envelope Antibody (biotin)

Infectious Disease, COVID-19

     
  • 10 - SARS-CoV-2 (COVID-19) Envelope Antibody (biotin) ASC12229
    Figure 1 ELISA Validation
    Coating Antigen: immunogen peptide, 3531P, 10 μg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, ASC12229 or 3531, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. ASC12229 was detected by HRP-conjugated streptavidin at 1:5,000 and 3531 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10,000 , incubate at RT for 1 hr.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
E
Primary Accession QHD43418
Other Accession QHD43418
Host Rabbit
Clonality Polyclonal
Isotype IgG
Clone Names E
Concentration (mg/ml) 1 mg/mL
Conjugate Biotin
Additional Information
Gene ID 43740570
Other Names SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Envelope protein, E protein
Reconstitution & Storage SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsSARS-CoV-2 (COVID-19) Envelope Antibody (biotin) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Research Areas

BACKGROUND

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Envelope protein is a small polypeptide that contains at least one alpha-helical transmembrane domain. It involves in several aspects of the virus's life cycle, such as assembly, budding, envelope formation, and pathogenesis. E protein has membrane permeabilizing activity, which provides a possible rationale to inhibit in vitro ion channel activity of some synthetic coronavirus E proteins, and also viral replication (3).

REFERENCES

Gorbalenya. bioRxiv: 2020.;Hui et al. Int J Infect Dis. 2020;91:264-266.;Pervushin et al. PLoS Pathog. 2009; 5(7): e1000511.

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