癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Control Mouse IgG For Western Blot
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- 背景知识
| Description | Control Mouse IgG for Western Blot |
|---|---|
| Conjugate | Unconjugated |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Stabilizer | 50% (v/v) Glycerol |
| Preservative | 0.01% (w/v) Sodium Azide and 0.01% (w/v) Gentamicin Sulfate |
| Shipping Condition | Ambient |
|---|---|
| Application Note | This product is a normal IgG control for use with Rockland Colorimetric Western Blot Kit: TMB/DAB Substrate Kit for Western Blot using Mouse Primary Antibody (Catalog number KIA002). See Kit insert for complete instructions. |
| Purity | This product was prepared from normal Mouse serum by a multi-stage process which includes delipidation, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum. |
| Storage Condition | See kit insert for complete instructions. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This western blotting kit allows for the detection of primary mouse polyclonal or monoclonal antibody provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. The first step in the detection of the membrane bound primary antibody-antigen complex is the addition of a secondary antibody that has been affinity purified, cross-adsorbed, and biotinylated. This biotinylated secondary antibody is visualized after reaction with streptavidin-peroxidase conjugate and subsequent addition of either TMB or DAB substrates. The increased sensitivity and low background achieved by Rockland Immunochemicals' Western Blotting Kit relies on the use of an enhanced streptavidin-peroxidase conjugate and a highly active biotinylated antibody. The streptavidin carries a neutral charge and fewer carbohydrate groups than positively charged avidin. The biotin incorporates a ''spacer'' group that allows for better accessibility of the streptavidin-peroxidase conjugate.
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