癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
HRP Western Blot Anti-Mouse IgG Antibody
Goat Polyclonal, Peroxidase (Horseradish)
- 产品详情
- 实验流程
- 背景知识
| Description | HRP Western Blot Anti-Mouse IgG Antibody |
|---|---|
| Host | Goat |
| Conjugate | Peroxidase (Horseradish) |
| Clonality | Polyclonal |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Wet Ice |
|---|---|
| Application Note | Western Blot Analysis |
| Storage Condition | See kit insert for complete instructions. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
HRP is used in applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal and HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. Chemiluminescence is the best all-around method for western blot detection as these systems eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. These methods are also unmatched in the speed and sensitivity compared to traditional alternatives, utilizing film to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. This item is a component of kit (KCA002). The Kit contains all the reagents necessary to perform chemiluminescent Western Blots to detect mouse primary antibodies.
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