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>   首页   >   产品   >   一抗   >   其他   >   Anti-Chicken IgG (H&L) Antibody (Peroxidase Conjugated)   

Anti-Chicken IgG (H&L) Antibody (Peroxidase Conjugated)

Polyclonal, Peroxidase (Horseradish)

     
  • 1 - Anti-Chicken IgG (H&L) Antibody (Peroxidase Conjugated) ASR1085
    Western Blot of Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (RABBIT) Antibody (p/n 603-4102) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.
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Product Information
Description Anti-CHICKEN IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated
Conjugate Peroxidase (Horseradish)
Target Species Chicken
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Chicken IgG whole molecule
Reconstitution Volume 500 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Application Note Anti-Chicken IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc againstanti-Peroxidase, anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Chicken IgG antibody generated in rabbit detects specifically chicken IgG. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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