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Anti-Sheep (Peroxidase Conjugated) IgG [H&L] (Pre-Adsorbed) Secondary Antibody
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| Description | Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG [H&L] (RABBIT) (Min X Human Serum Proteins) |
|---|---|
| Host | Rabbit |
| Conjugate | Peroxidase (Horseradish) |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Sheep IgG whole molecule |
| Reconstitution Volume | 500 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Ambient |
|---|---|
| Application Note | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other enzymatic assays requiring lot-to-lot consistency. |
| Purity | Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins. |
| Storage Condition | Store secondary antibody 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. HRP Anti-IgG Secondary Antibody conjugate is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency
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