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Mouse IgG1 isotype control Biotin

Monoclonal MG1 IgG1 , Biotin

     
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Product Information
Description MOUSE IgG1 isotype control Biotin conjugated
Conjugate Biotin
Clonality Monoclonal MG1 IgG1
Physical State Lyophilized
Host Isotype IgG1
Buffer 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Species of Origin Mouse
Reconstitution Volume 100 µL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Application Note Mouse IgG1 isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Purity This product has been prepared from in vitro cell culture by selective precipitation.  In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Typically, less than 1% cross reactivity was detected by ELISA against other mouse and human heavy or light chains isotypes using chain specific antibodies.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Isotype control Mouse IgG1 is important for Flow Cytometry. Mouse IgG1 control has no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.

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