Anti-Chicken IgG (H&L) Pre-Adsorbed Secondary Antibody
Donkey Polyclonal, Unconjugated
- 产品详情
- 实验流程
- 背景知识
Description | Anti-CHICKEN IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) |
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Host | Donkey |
Conjugate | Unconjugated |
Target Species | Chicken |
Reactivity | Chicken |
Clonality | Polyclonal |
Application ![]()
| WB |
Physical State | Liquid (sterile filtered) |
Host Isotype | IgG |
Target Isotype | IgG (H&L) |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Anti-Chicken IgY (H&L) was produced by repeated immunization with Chicken IgY whole molecule in donkey. |
Stabilizer | None |
Preservative | None |
Shipping Condition | Dry Ice |
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Application Note | Anti-Chicken IgY (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
Purity | Anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgY and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins. |
Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Chicken IgY antibody generated in donkey detects specifically chicken IgG. This anti-Chicken secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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