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Human Transferrin Biotin

     
  • 6 - Human Transferrin Biotin  ASR1282
    Dot Blot of Biotin conjugated Human Transferrin. Antigen: Human Transferrin Biotin. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 60 min at RT.
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Product Information
Description HUMAN TRANSFERRIN Biotin conjugated
Conjugate Biotin
FP Value 10-20 moles Biotin per mole of Human Transferrin
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB
Physical State Lyophilized
Host Isotype Transferrin
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Human
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/ml Polyethylene Glycol (PEG-8000)
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Application Note Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
Purity This product was prepared from normal serum by a multi-stage process that includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human Transferrin and anti-Human Serum.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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