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Anti-Rabbit IgG (H&L) (Rhodamine Conjugated) Secondary Antibody

Goat Polyclonal, Rhodamine (TRITC)

     
  • 1 - Anti-Rabbit IgG (H&L)  (Rhodamine Conjugated) Secondary Antibody ASR1424
    Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
  • 1 - Anti-Rabbit IgG (H&L)  (Rhodamine Conjugated) Secondary Antibody ASR1424
    Western Blot of Goat anti-Rabbit IgG Rhodamine Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
  • 6 - Anti-Rabbit IgG (H&L)  (Rhodamine Conjugated) Secondary Antibody ASR1424
    Dot Blot of Rhodamine Conjugated Goat-anti-Rabbit IgG. Antigen: Rabbit IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng. Primary antibody: none. Secondary antibody: Rhodamine Conjugated Goat-a-Rabbit IgG secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.
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Product Information
Description Anti-RABBIT IgG (H&L) (GOAT) Antibody Rhodamine Conjugated
Host Goat
Conjugate Rhodamine (TRITC)
FP Value 3.6 moles Rhodamine (TRITC) per mole of IgG
Target Species Rabbit
Reactivity Rabbit
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, DB
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rabbit IgG whole molecule
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

BACKGROUND

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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