癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Mouse IgG2a isotype control Peroxidase
Monoclonal M2A IgG2a , Peroxidase (Horseradish)
- 产品详情
- 实验流程
- 背景知识
| Description | MOUSE IgG2a isotype control Peroxidase conjugated |
|---|---|
| Conjugate | Peroxidase (Horseradish) |
| Clonality | Monoclonal M2A IgG2a |
| Physical State | Lyophilized |
| Host Isotype | IgG2a |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Species of Origin | Mouse |
| Reconstitution Volume | 100 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Ambient |
|---|---|
| Application Note | Mouse IgG2a isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. |
| Purity | This product has been prepared from immunodeficient mouse ascites by protein A chromatography using specific conditions for subclass purification. Typically, less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Isotype controls are important for Flow Cytometry and have no specificity for target cells within a particular experiment. Their purpose is to confirm the specificity of primary antibody binding that it is not a result of non-specific Fc receptor binding to cells or other cellular protein interactions. Isotype controls need to be matched to the specific primary Abs (species and isotype, including heavy and light chains) being used.
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