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Rat IgG Fab Texas Red™

     
  • 0 - Rat IgG Fab Texas Red™  ASR1508
    SDS-PAGE of Rat IgG F(ab) Fragment Texas Red™ Conjugated (p/n ASR1508). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rat IgG Whole Molecule (p/n 012-0102). Lane 2: Reduced Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Reduced Rat IgG F(ab) Fragment Texas Red™ Conjugated (p/n ASR1508). Lane 4: Reduced Rat IgM Whole Molecule (p/n 012-0107). Load: 1 µg of IgG, F(c), F(ab); 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
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Product Information
Description RAT IgG F(ab) fragment Texas Red™ conjugated
Conjugate Texas Red®
FP Value 2.1 moles Texas Red® per mole of Rat IgG F(ab)
Physical State Lyophilized
Host Isotype IgG F(ab)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Rat
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum.  No reaction was observed against anti-Rat IgG F(c) or anti-Papain.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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