注册 | 登录
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   其他   >   Anti-Rabbit IgG F(c) Secondary Antibody   

Anti-Rabbit IgG F(c) Secondary Antibody

Goat Polyclonal, Unconjugated

     
  • 1 - Anti-Rabbit IgG F(c)  Secondary Antibody ASR1631
    Western Blot of Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n ASR1631). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng of IgG, F(ab), IgM and Serum, 100 ng of F(c). Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(c) (GOAT) Antibody (p/n ASR1631) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
  • 1 - Anti-Rabbit IgG F(c)  Secondary Antibody ASR1631
    Western Blot of Goat Anti-Rabbit F(c) secondary antibody. Lane 1: Rabbit F(c). Lane 2: None. Load: 50 ng per lane. Primary antibody: Rabbit F(c) antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:20,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 kDa, 25 kDa for Rabbit F(c). Other band(s): None.
  • 产品详情
  • 实验流程
  • 背景知识
Product Information
Description Anti-RABBIT IgG F(c) (GOAT) Antibody
Host Goat
Conjugate Unconjugated
Target Species Rabbit
Reactivity Rabbit
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG F(c)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG f(c) fragment in goat
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Application Note Antibody Anti-Rabbit IgG F(c) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab’)2.
Storage Condition Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Rabbit antibody generated in goat detects specifically rabbit IgG F(s). This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价