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Anti-RABBIT IgG (H&L) (Peroxidase Conjugated) Secondary Antibody

Goat Polyclonal, Peroxidase (Horseradish)

     
  • 1 - Anti-RABBIT IgG (H&L)  (Peroxidase Conjugated) Secondary Antibody ASR1633
    Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH/3T3 Whole Cell Lysate. Load: 10 µg per lane. Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 42 kDa, 42 kDa.
  • 1 - Anti-RABBIT IgG (H&L)  (Peroxidase Conjugated) Secondary Antibody ASR1633
    Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (p/n 611-1102) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
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Product Information
Description Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated
Host Goat
Conjugate Peroxidase (Horseradish)
Target Species Rabbit
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rabbit IgG whole molecule
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Purity RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage Condition Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

BACKGROUND

Rabbit secondary antibody conjugated to horseradish peroxidase (HRP) is available in a variety of formats. HRP Anti IgG secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other HRP antibody based assays.

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