Anti-Sheep IgG F(c) Secondary Antibody
Rabbit Polyclonal, Unconjugated
- 产品详情
- 实验流程
- 背景知识
| Description | Anti-SHEEP IgG F(c) (RABBIT) Antibody |
|---|---|
| Host | Rabbit |
| Conjugate | Unconjugated |
| Target Species | Sheep |
| Reactivity | Sheep |
| Clonality | Polyclonal |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG |
| Target Isotype | IgG F(c) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit. |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Wet Ice |
|---|---|
| Application Note | Anti-Sheep IgG F(c) antibody has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. |
| Purity | Anti-Sheep IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab’)2. |
| Storage Condition | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Anti-Sheep IgG F(c) antibody generated in rabbit detects specifically sheep IgG F(c). Anti-Sheep IgG F(c) secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.Anti-Sheep IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
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