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Anti-Sheep IgG F(c) Secondary Antibody

Rabbit Polyclonal, Unconjugated

     
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Product Information
Description Anti-SHEEP IgG F(c) (RABBIT) Antibody
Host Rabbit
Conjugate Unconjugated
Target Species Sheep
Reactivity Sheep
Clonality Polyclonal
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG F(c)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Application Note Anti-Sheep IgG F(c) antibody has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Purity Anti-Sheep IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum.  No reaction was observed against Sheep IgG F(ab’)2.
Storage Condition Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Sheep IgG F(c) antibody generated in rabbit detects specifically sheep IgG F(c). Anti-Sheep IgG F(c) secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.Anti-Sheep IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.

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