Anti-Rat IgM (mu chain) Secondary Antibody
Goat Polyclonal, Unconjugated
- 产品详情
- 实验流程
- 背景知识
Description | Anti-RAT IgM (mu chain) (GOAT) Antibody |
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Host | Goat |
Conjugate | Unconjugated |
Target Species | Rat |
Clonality | Polyclonal |
Application ![]()
| WB |
Physical State | Liquid (sterile filtered) |
Host Isotype | IgG |
Target Isotype | IgM µ chain |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Rat IgM mu heavy chain |
Stabilizer | None |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Wet Ice |
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Application Note | Anti-RAT IgM (mu chain) (GOAT) Antibody has been assayed against Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Rat IgM antibody is suitable for immunosassays including western blot and ELISA. |
Purity | Anti-RAT IgM (mu chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgG or Rat IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other rat heavy or light chain isotypes. |
Storage Condition | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Rat IgM antibody recognizes the mu chain of the Rat IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgA or Rat IgG. ELISA was used to confirm specificity at less than 1% cross reactivity against other rat heavy or light chain isotypes. Anti-Rat IgM antibody is ideal with investigators involved in serum component protein research.

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