Anti-MOUSE IgG (H&L) (Peroxidase Conjugated) Secondary Antibody
Rabbit Polyclonal, Peroxidase (Horseradish)
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Description | Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated |
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Host | Rabbit |
Conjugate | Peroxidase (Horseradish) |
Target Species | Mouse |
Clonality | Polyclonal |
Application ![]()
| WB |
Physical State | Lyophilized |
Host Isotype | IgG |
Target Isotype | IgG (H&L) |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Mouse IgG whole molecule |
Reconstitution Volume | 1.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Shipping Condition | Ambient |
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Application Note | When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment |
Purity | HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. |
Storage Condition | Store mouse second antibody conjugate at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Mouse secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is Suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

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