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Anti-Armenian Hamster IgG (H&L) (Fluorescein Conjugated) Secondary Antibody

Goat Polyclonal, Fluorescein (FITC)

     
  • 1 - Anti-Armenian Hamster IgG (H&L) (Fluorescein Conjugated)  Secondary Antibody ASR1960
    Western Blot of Fluorescein conjugated Goat anti-Armenian Hamster IgG secondary antibody. Lane 1: Armenian Hamster IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Armenian Hamster IgG. Other band(s): none.
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Product Information
Description Anti-ARMENIAN HAMSTER IgG (H&L) (GOAT) Antibody Fluorescein Conjugated
Host Goat
Conjugate Fluorescein (FITC)
FP Value 2.8 moles Fluorescein (FITC) per mole of IgG
Target Species Armenian Hamster
Reactivity Hamster
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Armenian Hamster IgG whole molecule
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum.  Minimal reactivity will occur against Golden Syrian Hamster IgG.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

FITC Anti-Hamster IgG Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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