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F(ab')2 Anti-Guinea Pig IgG (H&L) (Fluorescein Conjugated) Secondary Antibody
Goat Polyclonal, Fluorescein (FITC)
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| Description | F(ab')2 Anti-GUINEA PIG IgG (H&L) (GOAT) Antibody Fluorescein Conjugated |
|---|---|
| Host | Goat |
| Conjugate | Fluorescein (FITC) |
| FP Value | 1.7 moles Fluorescein (FITC) per mole of IgG F(ab')2 |
| Target Species | Guinea Pig |
| Clonality | Polyclonal |
| Physical State | Lyophilized |
| Host Isotype | IgG F(ab')2 |
| Target Isotype | IgG (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Guinea Pig IgG whole molecule |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Application Note | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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