癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Human IgM (mu chain) (Peroxidase Conjugated) Secondary Antibody
Goat Polyclonal, Peroxidase (Horseradish)
- 产品详情
- 实验流程
- 背景知识
| Description | Anti-HUMAN IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated |
|---|---|
| Host | Goat |
| Conjugate | Peroxidase (Horseradish) |
| Target Species | Human |
| Clonality | Polyclonal |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgM µ chain |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Human IgM mu heavy chain |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Ambient |
|---|---|
| Application Note | Human IgM (mu chain) Antibody is peroxidase conjugated and is suitable for immunoassays where specificity to the immunoglobulin mu chain region is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemisty. Optimal concentrations in immunoassays should be determined by the researcher. |
| Purity | Human IgM (mu chain) Antibody, Peroxidase Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA minimal cross-reactivity against other human heavy or light chain isotypes. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Human IgM (mu chain) Antibody, Peroxidase Conjugated detects IgM. Immunoglobulin M, or IgM, is an antibody produced by B cells. IgM is a pentamer composed of 5 immunogobulin molecules linked through their F(c) domain by the J chain. The mu chain is the heavy chain of IgM and contains an F(c) domain. IgM is the first antibody to appear in response to initial exposure to antigen, followed by IgG. The spleen is the major site of specific IgM production, IgM in normal serum is often found to bind to specific antigens , even in the absence of prior immunization making it a first line of immune surveillance.
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