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Anti-Mouse IgG + IgM (H&L) (Rhodamine Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Rhodamine (TRITC)
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| Description | Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody Rhodamine Conjugated Minimum Cross Reactivity to Bovine, Horse and Human Serum Proteins |
|---|---|
| Host | Goat |
| Conjugate | Rhodamine (TRITC) |
| FP Value | 3.3 moles Rhodamine (TRITC) per mole of Mouse IgG |
| Target Species | Mouse |
| Clonality | Polyclonal |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG + IgM (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Mouse IgG and IgM whole molecules |
| Species of Origin | Mouse |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Application Note | A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Mouse IgM antibody is suitable for immunosassays including western blot and ELISA. |
| Purity | Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Anti-MOUSE IgG + IgM (H&L) (GOAT) Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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