Anti-Human IgG F(c) (Biotin Conjugated) Secondary Antibody
Rabbit Polyclonal, Biotin
- 产品详情
- 实验流程
- 背景知识
Description | Anti-HUMAN IgG F(c) (RABBIT) Antibody Biotin Conjugated |
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Host | Rabbit |
Conjugate | Biotin |
Target Species | Human |
Reactivity | Human |
Clonality | Polyclonal |
Physical State | Lyophilized |
Host Isotype | IgG |
Target Isotype | IgG F(c) |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Immunogen | Anti-Human IgG F(c) was produced by repeated immunization with human IgG F(c) fragment in rabbit. |
Reconstitution Volume | 1.0 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Ambient |
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Application Note | Anti-Human IgG F(c) Biotin conjugated antibodies are suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab’)2. |
Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Anti-Human F(c) biotin conjugated antibody generated in rabbit detects specifically human IgG F(c). This secondary biotin conjugated antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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