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Anti-MOUSE IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody

Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)

     
  • 1 - Anti-MOUSE IgG (H&L)  (Alkaline Phosphatase Conjugated) Secondary Antibody ASR2208
    Western Blot of Rabbit anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated Lane 1: Mouse IgG Load: 100 ng per lane Secondary antibody: MOUSE IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa
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Product Information
Description Anti-MOUSE IgG (H&L) (RABBIT) Antibody Alkaline Phosphatase Conjugated
Host Rabbit
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Mouse IgG whole molecule
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Application Note Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Purity Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Storage Condition Store secondary antibody at 4° C before opening. DO NOT FREEZE. Mouse conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Conjugated Secondary Antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.

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