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Anti-Monkey IgM (mu chain) (Fluorescein Conjugated) Secondary Antibody

Goat Polyclonal, Fluorescein (FITC)

     
  • 1 - Anti-Monkey IgM (mu chain)  (Fluorescein Conjugated) Secondary Antibody ASR2237
    Western Blot of Fluorescein conjugated Goat Anti-Monkey IgM (mu chain) secondary antibody. Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None.
  • 0 - Anti-Monkey IgM (mu chain)  (Fluorescein Conjugated) Secondary Antibody ASR2237
    This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.
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Product Information
Description Anti-MONKEY IgM (mu chain) (GOAT) Antibody Fluorescein Conjugated
Host Goat
Conjugate Fluorescein (FITC)
FP Value 3.0 moles Fluorescein (FITC) per mole of IgG
Target Species Monkey
Clonality Polyclonal
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgM µ chain
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Monkey IgM mu heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Monkey IgM and Monkey Serum.  No reaction was observed against Monkey IgG or Monkey IgA.  Specificity was confirmed by ELISA at less than 1% cross-reactivity against other Monkey heavy or light chain isotypes.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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