Rat IgG Alkaline Phosphatase

SDS-PAGE of Rat IgG Whole Molecule Alkaline Phosphatase Conjugated (p/n ASR2276). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rat IgG Whole Molecule Alkaline Phosphatase Conjugated (p/n ASR2276). Lane 2: Reduced Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Reduced Rat IgG F(ab) Fragment (p/n 012-0105). Lane 4: Reduced Rat IgM Whole Molecule (p/n 012-0107). Load: 1 µg of IgG, F(c), F(ab); 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.

Product Information
Description	RAT IgG whole molecule Alkaline Phosphatase conjugated
Conjugate	Alkaline Phosphatase (Calf Intestine)
Physical State	Liquid (sterile filtered)
Host Isotype	IgG
Buffer	0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Species of Origin	Rat

Additional Information
Shipping Condition	Wet Ice
Purity	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG, anti-Rat Serum and anti-Alkaline Phosphatase (calf intestine).
Storage Condition	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.