Rat IgG Fab

SDS-PAGE of Rat IgG F(ab) Fragment (p/n ASR2307). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rat IgG Whole Molecule (p/n 012-0102). Lane 2: Reduced Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Reduced Rat IgG F(ab) Fragment (p/n ASR2307). Lane 4: Reduced Rat IgM Whole Molecule (p/n 012-0107). Load: 1 µg of IgG, F(c), F(ab); 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.

Product Information
Description	RAT IgG F(ab) fragment
Conjugate	Unconjugated
Physical State	Liquid (sterile filtered)
Host Isotype	IgG F(ab)
Buffer	0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin	Rat
Stabilizer	None
Preservative	0.01% (w/v) Sodium Azide

Additional Information
Shipping Condition	Wet Ice
Application Note	Suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins.
Purity	This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(ab’)2. No reaction was observed against anti-Rat IgG F(c) or anti- Papain.
Storage Condition	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.