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Anti-Human IgM (Fc5µ) Secondary Antibody
Mouse Monoclonal 22G1.H8 IgG2b kappa , Unconjugated
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- 背景知识
| Description | Anti-HUMAN IgM (Fc5 µ) (MOUSE) Antibody |
|---|---|
| Host | Mouse |
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Monoclonal 22G1.H8 IgG2b kappa |
Application 
| WB |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG2b |
| Target Isotype | IgM Fc5 µ |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Human IgM Fc5 µ was produced by repeated immunization with Human IgM Fc5 µ fragment in mice. |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Dry Ice |
|---|---|
| Application Note | Human IgM Fc5 µ antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. |
| Purity | Anti-Human IgM Fc5 µ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5 µ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human F(ab) or Human F(ab)2. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Human IgM Fc5 µ antibody generated in mice detects specifically human IgM. his anti-human IgM secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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