Anti-Rat-IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody
Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)
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Description | Anti-RAT-IgG (H&L) (RABBIT) Antibody Alkaline Phosphatase Conjugated |
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Host | Rabbit |
Conjugate | Alkaline Phosphatase (Calf Intestine) |
Target Species | Rat |
Clonality | Polyclonal |
Physical State | Liquid (sterile filtered) |
Host Isotype | IgG |
Target Isotype | IgG (H&L) |
Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
Immunogen | Rat IgG whole molecule |
Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Wet Ice |
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Application Note | When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment |
Purity | Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG and Rat Serum. |
Storage Condition | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency

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