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F(ab')2 Anti-Mouse IgG IgA IgM (H&L) (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Peroxidase (Horseradish)
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| Description | F(ab')2 Anti-MOUSE IgG IgA IgM (H&L) (GOAT) Antibody Peroxidase Conjugated Min X Human Serum Proteins |
|---|---|
| Host | Goat |
| Conjugate | Peroxidase (Horseradish) |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
| Physical State | Lyophilized |
| Host Isotype | IgG F(ab')2 |
| Target Isotype | IgG IgA IgM |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Mouse IgG IgA and IgM (H&L) was produced by repeated immunization with mouse IgG IgA and IgM molecule in goat. |
| Reconstitution Volume | 500 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Thimerosal |
| Shipping Condition | Ambient |
|---|---|
| Application Note | Antibodies Anti-Mouse IgG IgA IgM peroxidase conjugated are suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. |
| Purity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c). This reagent is suitable for the detection of all mouse subclasses, isotypes and chain combinations. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Anti-Mouse IgG IgA IgM peroxidase conjugated antibody generated in goat detect specifically mouse IgG. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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