Goat IgM

SDS-PAGE of Goat IgM Whole Molecule (p/n ASR2561). Lane M: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Goat IgG Whole Molecule (p/n 005-0102). Lane 2: Reduced Goat IgG F(c) Fragment (p/n 005-0103). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n 005-0105). Lane 4: Reduced Goat IgM Whole Molecule (p/n ASR2561). Load: 1 µg for IgG, F(c) and F(ab); 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Product Information
Description	GOAT IgM whole molecule
Conjugate	Unconjugated
Physical State	Liquid (sterile filtered)
Host Isotype	IgM
Buffer	0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Species of Origin	Goat
Stabilizer	10% (v/v) Glycerol
Preservative	0.1% (w/v) Sodium Azide

Additional Information
Shipping Condition	Wet Ice
Purity	This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and anti-Goat IgM ( µ chain specific). No reaction was observed against anti-Goat IgG F(c). Some light chain cross reactivity will occur with anti-Goat IgG.
Storage Condition	Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.