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Anti-MOUSE IgG2a (Peroxidase Conjugated) Pre-adsorbed Secondary Antibody

Goat Polyclonal, Peroxidase (Horseradish)

     
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Product Information
Description Anti-MOUSE IgG2a (Gamma 2a chain) (GOAT) Antibody Peroxidase Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins)
Host Goat
Conjugate Peroxidase (Horseradish)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG2a
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgG2a heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Application Note Anti-Mouse IgG2a Peroxidase Secondary antibodies are suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays.
Purity MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Storage Condition Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Mouse IgG2a secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment compositions.

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