Anti-Mouse IgG3 (Peroxidase Conjugated) Pre-adsorbed Secondary Antibody
Goat Polyclonal, Peroxidase (Horseradish)
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| Description | Anti-MOUSE IgG3 (Gamma 3 chain) (GOAT) Antibody Peroxidase Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins) |
|---|---|
| Host | Goat |
| Conjugate | Peroxidase (Horseradish) |
| Target Species | Mouse |
| Reactivity | Mouse |
| Clonality | Polyclonal |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG3 |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Mouse IgG3 heavy chain |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Ambient |
|---|---|
| Application Note | Anti-Mouse IgG3 peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods and anti IgG2b antibody based assays. |
| Purity | Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Mouse IgG3 secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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