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F(ab')2 Anti-Mouse IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)
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| Description | F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated Min X HUMAN Serum Proteins |
|---|---|
| Host | Goat |
| Conjugate | Alkaline Phosphatase (Calf Intestine) |
| Target Species | Mouse |
| Clonality | Polyclonal |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG F(ab')2 |
| Target Isotype | IgG (H&L) |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Immunogen | Mouse IgG whole molecule |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Wet Ice |
|---|---|
| Application Note | This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins. |
| Storage Condition | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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