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Rabbit IgG F(c) Rhodamine

     
  • 0 - Rabbit IgG F(c) Rhodamine  ASR2835
    SDS-PAGE of Rabbit IgG F(c) Fragment Rhodamine Conjugated (p/n ASR2835). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rabbit IgG Whole Molecule (p/n 011-0102). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Reduced Rabbit IgG F(c) Fragment Rhodamine Conjugated (p/n ASR2835). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n 011-0107). Load: 1 µg for F(ab) and F(c); 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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Product Information
Description RABBIT IgG F(c) fragment Rhodamine conjugated
Conjugate Rhodamine (TRITC)
Physical State Lyophilized
Host Isotype IgG F(c)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Rabbit
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG, anti-Rabbit IgG F(c) and anti-Rabbit Serum.  No reaction was observed against anti-Rabbit IgG F(ab’)2 or anti-Papain.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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