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F(ab')2 Anti-Mouse IgG (H&L) (Biotin Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Biotin
- 产品详情
- 实验流程
- 背景知识
| Description | F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody Biotin Conjugated Min X Bv Hs Hu Rb Rt & Sh Serum Proteins |
|---|---|
| Host | Goat |
| Conjugate | Biotin |
| Target Species | Mouse |
| Clonality | Polyclonal |
| Physical State | Lyophilized |
| Host Isotype | IgG F(ab')2 |
| Target Isotype | IgG (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat. |
| Reconstitution Volume | 500 µL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Application Note | F(ab')2 Anti-Mouse IgG (H&L) (GOAT) Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
| Purity | F(ab')2 Anti-Mouse IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
F(ab')2 anti-Mouse IgG (H&L) antibody generated in goat detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.F(ab')2 Anti-Mouse IgG (H&L) (GOAT) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
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