Chicken IgG F(c)

SDS-PAGE of Chicken IgG F(c) Fragment (p/n ASR2895). Lane M: 5 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Chicken IgG Whole Molecule (p/n 003-0102). Lane 2: Reduced Chicken IgG F(c) Fragment (p/n ASR2895). Lane 3: Reduced Chicken IgG F(ab) Fragment (p/n 003-0105). Lane 4: Reduced Chicken IgM Whole Molecule (p/n 003-0107). Load: 1 µg per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.

Product Information
Description	CHICKEN IgG F(c) fragment
Conjugate	Unconjugated
Physical State	Lyophilized
Host Isotype	IgG F(c)
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin	Chicken
Stabilizer	None
Preservative	0.01% (w/v) Sodium Azide

Additional Information
Shipping Condition	Wet Ice
Purity	This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, anti-Chicken IgG and anti-Chicken IgG F(c). No reaction was observed against anti-Chicken IgG F(ab’)2 or anti-Papain.
Storage Condition	Store vial at 4° C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions Note	This product is for research use only and is not intended for therapeutic or diagnostic applications.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.