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Anti-Mouse IgM (H&L) (Peroxidase Conjugated) Secondary Antibody

Goat Polyclonal, Peroxidase (Horseradish)

     
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Product Information
Description Anti-MOUSE IgM (H&L) (GOAT) Antibody Peroxidase Conjugated
Host Goat
Conjugate Peroxidase (Horseradish)
Target Species Mouse
Clonality Polyclonal
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgM (H&L)
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Mouse IgM secondary antibody was produced by repeated immunization with Mouse IgM whole molecule in rabbit.
Reconstitution Volume 2.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Application Note Anti-Mouse IgM antibody is sutiable for use in various assays. Optimal dilutions for use should be determined by the user.
Purity Anti-Mouse IgM secondary antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.  This reagent primarily recognizes Mouse IgM however some light chain cross reactivity will occur against other mouse immunoglobulins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Mouse IgM antibody generated in rabbit detects specifically Mouse IgM with some light chain cross reactivity against Mouse IgG. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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