癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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Anti-Rabbit IgM (mu) (Peroxidase Conjugated) Secondary Antibody
Goat Polyclonal, Peroxidase (Horseradish)
- 产品详情
- 实验流程
- 背景知识
| Description | Anti-RABBIT IgM (mu) (GOAT) Antibody Peroxidase Conjugated |
|---|---|
| Host | Goat |
| Conjugate | Peroxidase (Horseradish) |
| Target Species | Rabbit |
| Reactivity | Rabbit |
| Clonality | Polyclonal |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgM µ chain |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Rabbit IgM (mu) was produced by repeated immunization with rabbit IgM mu heavy chain in goat. |
| Reconstitution Volume | 2.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Ambient |
|---|---|
| Application Note | Rabbit IgM (mu) peroxidase conjugated antibody has been assayed against 1.0 ug of Rabbit IgM in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product. |
| Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgM and Rabbit Serum. No reaction was observed against Rabbit IgG. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Anti-Rabbit IgM (mu) peroxidase conjugated antibody generated in goat detects specifically rabbit IgM (mu). This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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