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Anti-Mouse IgM (mu chain) (Peroxidase Conjugated) Secondary Antibody

Rabbit Polyclonal, Peroxidase (Horseradish)

     
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Product Information
Description Anti-MOUSE IgM (mu chain) (RABBIT) Antibody Peroxidase Conjugated
Host Rabbit
Conjugate Peroxidase (Horseradish)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgM µ chain
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Mouse IgM (mu chain) was produced by repeated immunization with mouse IgM mu heavy chain in rabbit.
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Application Note Mouse IgM (mu chain) peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Purity Anti-Mouse IgM HRP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgM and Mouse Serum.   Specificity was confirmed by ELISA at less than 1% cross reactivity against other Mouse heavy or light chain isotypes.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Mouse IgM (mu chain) peroxidase conjugated antibody generated in rabbit detects specifically mouse IgM. This Mouse IgM secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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