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>   首页   >   产品   >   一抗   >   其他   >   F(ab')2 Anti-Rat IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody   

F(ab')2 Anti-Rat IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody

Donkey Polyclonal, R-Phycoerythrin (RPE)

     
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Product Information
Description F(ab')2 Anti-RAT IgG [H&L] (DONKEY) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins
Host Donkey
Conjugate R-Phycoerythrin (RPE)
Target Species Rat
Clonality Polyclonal
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgG whole molecule
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Application Note Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.  Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rat IgG and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Sheep Serum Proteins.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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