癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Leptin Secondary Antibody
Rabbit Polyclonal, Unconjugated
- 产品详情
- 实验流程
| Description | Anti-Leptin (RABBIT) Antibody |
|---|---|
| Host | Rabbit |
| Conjugate | Unconjugated |
| Target Species | Mouse |
| Reactivity | Human, Mouse |
| Clonality | Polyclonal |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | Antiserum |
| Buffer | None |
| Immunogen | This whole rabbit serum was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli. |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Dry Ice |
|---|---|
| Application Note | This antiserum against Mouse Leptin has been tested for use in ELISA and immunoblotting. Reactivity in other immunoassays is unknown. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes.This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:2,400 is suggested for this product. |
| Purity | This antiserum has been heated to 56°C for 30 minutes. The antibody will recognize recombinant and native 16 kDa leptin from mouse and human. Reactivity with leptin from other sources is unknown. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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